Intro to Alignment Algorithms, Visualization, and QC
Key concepts
- Sequence alignment algorithms, BAM files, genome viewers, alignment quality assessment.
Learning objectives
- Compare and contrast DNA vs. RNA sequence alignment strategies
- Perform alignment of sequence data using a few popular alignment algorithms
- Explore the BAM file format and learn approaches for summarizing, filtering, and otherwise manipulating BAM files
- Learn to use a genome viewer
- Perform a quality assessment using the aligned data
Lecture
